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Figure 5 | BMC Molecular Biology

Figure 5

From: Cloning and characterization of cDNAs encoding putative CTCFs in the mosquitoes, Aedes aegypti and Anopheles gambiae

Figure 5

An. gambiae CTCF specifically binds the chicken 5'HS4 and Drosophila Fab8 insulator sequences. Sua4 cells were lysed and increasing amounts of total cell protein (1.5, 7.5, 15 μg represented as solid triangle) were incubated with radiolabeled insulator sequences as follows: A.) Drosophila Fab8 insulator sequence [19]; B.) chicken β-globin FII insulator sequence [6]. The complex was competed (Cp) with ~150-fold excess of cold, unlabeled probe DNA and supershifted (Ab) with polyclonal antibody sera raised against the C-terminal fragment of An. gambiae CTCF. The probe only lane is indicated by P.

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