Figure 3

Developmental expression profile of CTCF protein in Ae. aegypti and D. melanogaster. The expression of CTCF was analyzed using RNA isolated from multiple individuals at each of the indicated stages: E1 and E24 (embryos ≤ 1 hr and 24 hrs post-oviposition respectively), Lv (larvae), Pf (female pupae), Pm (male pupae), Pu (pupae), Af (adult females), Am (adult males), Ov- and Ov+ (ovaries from non-blood-fed and blood-fed females respectively). -RT, no reverse-transcriptase. A. and B.) Ae. aegypti CTCF, 20 cycles and 30 cycles respectively. C.) Ae. aegypti S17, 25 cycles. D.) D. melanogaster CTCF, 25 cycles. E.) D. melanogaster Rp49, 20 cycles.