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Figure 9 | BMC Molecular Biology

Figure 9

From: Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements

Figure 9

Effects of KLF15 on rhodopsin promoter. (A) 293 cells in 22 mm dishes were co-transfected with 2.5 μg of bovine rhodopsin (-130 to +70 bp)-luciferase fusion construct (bRho130-luc) together with the indicated amount (μg) of KLF15 expression vector. Luciferase activity (in relative light units) was corrected for transfection efficiency using β-GAL internal control. Bars show fold-change in relative luciferase expression compared to control transfections using expression vector lacking cDNA inserts. Error bars, 95% confidence interval. (B) same as (A) except 0.25 μg Crx expression vector was added. (C) Same as (A) except 0.25 μg Nrl expression vector was added. (D) Same as (A) except 0.25 μg Crx and 0.25 μg Nrl were added. Statistical differences from (A) control, (B) Crx alone; (C) Nrl alone; and (D) Crx+Nrl indicated by * p = 0.012, ** p < 0.008, *** p < 0.0001.

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