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Figure 7 | BMC Molecular Biology

Figure 7

From: Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements

Figure 7

DNAse I footprint analysis of KLF15 binding sites in the IRBP promoter. (A) A PCR fragment spanning -132 to +70 bp of the bovine IRBP promoter was end labeled with 33P at the -132 bp end (forward strand) and incubated with 250, 125 and 62.5 ng of purified KLF15-ZF fusion protein (lanes 2–4). Lanes 1 and 5 contained no fusion protein. Protected regions are designated by bars on the left of each panel with the nucleotide position of each protected region indicated. Previously identified protein binding sites are indicated by brackets. Transcription start site (+1) is indicated by an open arrowhead. (B) Same as (A) except the fragment was end-labeled with 33P at the +70 end (reverse strand). The bands present in KI-a (indicated by grey bar in Panel A) were also present in negative control lanes containing undigested probe (data not shown).

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BMC Molecular Biology

ISSN: 1471-2199

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