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Figure 4 | BMC Molecular Biology

Figure 4

From: Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements

Figure 4

Sequence specificity of KLF15 binding: mutational analysis of the bovine rhodopsin promoter element (bRho29). (A) Sequence of oligomers used in EMSA analysis showing mutations analyzed in B and C. (B) Graph summarizing results of competitive EMSA using 32P-labeled bRho29 and unlabeled mutant oligomers as indicated. B, bRho29 competitor; H, hRho20 competitor. Bars show % of shifted oligonucleotide competed by addition of cold competitor. Bars with negative value reflect an increase in the amount of oligonucleotide shifted following the addition of cold competitor. (C) Same as B, except 32P-labeled hRho29 oligonucleotide was used.

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BMC Molecular Biology

ISSN: 1471-2199

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