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Figure 3 | BMC Molecular Biology

Figure 3

From: Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements

Figure 3

Competitive EMSA using (A) 32P- bRho29 oligonuclotides (bovine). Lane 1, no protein; lane 2, GST protein control; lane 3, KLF15-ZF-GST without competitor; lanes 4 -11, same as lane 3 but with unlabeled competitors (50 ×, 250 × fold-excess) as indicated. Large arrows indicate specific bands; small arrow indicates non-specific band present in negative (no protein and GST only) controls; IRBP1, MOK2 site from bovine IRBP promoter (5'-GGACAGGATTAAAGGCTTACTGGAG-3'); IRBP2, MOK2 site from bovine IRBP intron1 (5'-GGACTTGTCAGGGCCTTTA-3')

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BMC Molecular Biology

ISSN: 1471-2199

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