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Figure 4 | BMC Molecular Biology

Figure 4

From: Nuclear distribution and chromatin association of DNA polymerase α-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast

Figure 4

The C-terminal fragment of Cdc23 S424 ::Tcs is not localized to the nucleus after cleavage by TEV protease. A. cdc23 S424 ::Tcs strain (P1323) was grown in plus (-TEV) or minus thiamine (+TEV) media at 25°C for 24 h, then shifted to 37°C for 4 h before fixation with methanol and acetone. Panels show Cdc23-CFP fluorescence and DAPI staining superimposed on phase images. Bar = 10 μm. B. Quantitation of experiment shown in A. 100 cells were counted for each time point.

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BMC Molecular Biology

ISSN: 1471-2199

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