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Table 4 Complementation of trz1

From: Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z

Constructs

Complement Δ trz1

ELAC2

No

ELAC2.FLAG

No

FLAG. ELAC2.C-term

No

ELAC2.N-term

No

ELAC2 (S217L)

No

ELAC2 (A541T)

No

ELAC2 (R781H)

No

ELAC2 (1641insG)

No

ELAC1

No

TRZ1 WT

Yes

FLAG.TRZ1 WT

Yes

trz1 M535T

Yes

FLAG.trz1 M535T

Yes

trz1 H542Ter

No

FLAG.trz1 H542Ter

No

trz1 G548R

No

FLAG.trz1 G648R

No

trz1 G229D

Yes

trz1 Q230A

Yes

trz1 G698D

No

trz1 D699A

No

FLAG.TRZ1. C-term

No

FLAG.TRZ1. N-term

No

FLAG.TRZ1- ELAC2

No

ELAC2-TRZ1

No

  1. The chimeric proteins are made by fusion of either the ELAC2 N-domain with TRZ1 C-domain or the reverse. Trz1p N-domain ends at a.a. 463 and the C-domain starts at a.a. 465. The ELAC2 N-domain ends at a.a. 481 and the C-domain starts at a.a. 481.
  2. The shuffle experiment is done in strain YL03-47 according to protocols described in [32]. The constructs are TRP -marked CEN vectors.