Figure 4
From: Investigation of factors responsible for cell line cytoplasmic expression differences
Pretreatment of COS-7 cells with T7 RNAP mRNA. Transfection of COS-7 cells with 0.75 μg of capped T7-RNAP mRNA for 24 hours, followed by a transfection with 0.75 μg of R011 (cytoplasmic) or L053 (nuclear) expression plasmids. Cells were harvested 24 h post plasmid transfection. Transfections and luciferase assays were performed as described in Materials and Methods. Error bars indicate standard error