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Figure 3 | BMC Molecular Biology

Figure 3

From: Investigation of factors responsible for cell line cytoplasmic expression differences

Figure 3

T7 RNAP is functional in COS-7 cells. Transfection of BHK or COS-7 cells with 0.75 μg of capped T7-RNAP mRNA for 24 hours, followed by a transfection with 0.75 μg of a luciferase reporter construct (L059) mRNA species. Cells were harvested 20 h post plasmid transfection. Transfections and luciferase assays were performed as described in Materials and Methods. Error bars indicate standard error. Note that in the absence of T7 RNAP (no pre-transfection) no luciferase was detected.

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