Figure 1
From: Investigation of factors responsible for cell line cytoplasmic expression differences
Autogene expression in COS-7 cells A) Diagrams of plasmids used. B) Comparison of cytoplasmic (R011) and nuclear (L053) expression plasmids in COS-7 cells. COS-7 cells were transfected with 0.75 μg/well of plasmid. Equimolar amounts of plasmids were added, and the total mass of DNA per transfection was kept equal by adding an unrelated plasmid (pPUC19). Transfections and luciferase assays were performed as described in Materials and Methods. Error bars indicate standard error.