TY - JOUR AU - Finn, Jonathan D. AU - Wong, Tabitha AU - Cullis, Pieter R. PY - 2005 DA - 2005/05/11 TI - Investigation of factors responsible for cell line cytoplasmic expression differences JO - BMC Molecular Biology SP - 11 VL - 6 IS - 1 AB - Previous work has described a novel cytoplasmic expression system that results in a 20-fold increase in the levels of gene expression over a standard CMV-based nuclear expression system, as compared with a 2–3 fold increase seen with previous similar systems. While this increase was seen with BHK and Neuro-2a cells, further studies revealed that some cell lines, such as COS-7, demonstrated relatively poor levels of cytoplasmic expression. The objective of this study was to determine what factors were responsible for the different expression levels between BHK (a high expressing cell line) and COS-7 (a low expressing cell line). SN - 1471-2199 UR - https://doi.org/10.1186/1471-2199-6-11 DO - 10.1186/1471-2199-6-11 ID - Finn2005 ER -