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Table 1 Quality assessment of the retrieved data. Quality assessment of the retrieved data. For the 2-ΔΔCT results the table shows inclination and R2 for ΔCT plotted against log RNA concentration and inclinations and R2 for CT plotted against log RNA concentration for eEF1α and LsTryp1. For the DART-PCR results the table shows PCR-efficiencies for eEF1α and LsTryp1 calculated by DART-PCR and the p-value (one-way ANOVA) for the hypothesis that there is no difference between the efficiencies.

From: Relative transcript quantification by Quantitative PCR: Roughly right or precisely wrong?

  2-ΔΔCT results DART-PCR results
  ΔCT vs. log [RNA] (R2) eEF1α CT vs. log [RNA] (R2) LsTryp1 CT vs. log [RNA] (R2) eEF1α LsTryp1 ANOVA
Unstarved -0.080 (0.16) -2.7664 (0.95) -2.7949 (0.99) 0.901 0.902 0.9863
Starved 0.065 (0.06) -2.8815 (0.99) -2.8171 (0.93) 0.839 0.491 0.0004
Refed -0.052 (0.07) -3.3109 (0.99) -3.3845 (0.99) 0.883 1.095 0.0004