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Figure 3 | BMC Molecular Biology

Figure 3

From: A minor alternative transcript of the fumarylacetoacetate hydrolase gene produces a protein despite being likely subjected to nonsense-mediated mRNA decay

Figure 3

Specificity of the anti-DEL100 antiserum. (A) The mouse antiserum was affinity purified on the recombinant C-terminal of DEL100 and used to probe the same membrane as in Figure 2B. The adsorbed fraction (top panel), specific of the C-terminal of DEL100, still recognizes the 31-kDa protein. The non-adsorbed fraction (bottom panel) shows no cross-reactivity to the protein. The star indicates a non-specific signal. (B) The DEL100-Myc protein was synthesized in a rabbit reticulocyte lysate and labeled with 35S (translation panel). The tagged protein is indicated by an arrow. The band below is the protein synthesized without the Myc tag. The anti-Myc was immobilized on protein A-sepharose and incubated with in vitro translated DEL100-Myc. Effectiveness of the immunoprecipitation was verified by blotting using the anti-Myc (1/2,000) antibody. The anti-DEL100 antiserum (1/1,000) recognizes the in vitro translated protein in the input (10% of the reaction) and the immunoprecipitate (IP, indicated by an arrow).

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BMC Molecular Biology

ISSN: 1471-2199

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