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Figure 2 | BMC Molecular Biology

Figure 2

From: A minor alternative transcript of the fumarylacetoacetate hydrolase gene produces a protein despite being likely subjected to nonsense-mediated mRNA decay

Figure 2

Del100 transcript encodes for a protein expressed in various tissues. (A) Schematic representation of the putative DEL100 and DEL231 proteins. FAH and DEL231 proteins are identical, except for the region encoded by exons 8 and 9, which is missing in DEL231. DEL100, due to the skipping of exon 8 and the resulting change in the coding frame, differs from FAH at its C-terminal end. The black bar below the DEL100 protein represents the region used to raise the antiserum. (B) 30 μg proteins of a 10% (w/v) tissue homogenate were separated on a 15% SDS-polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blotted using a rabbit anti-FAH (top panel; dilution 1:25,000) or a mouse anti-DEL100 antiserum (middle panel; dilution 1:1,000). A band with an apparent molecular weight of 31-kDa is detected by the anti-DEL100 antiserum in all tissues tested. The star indicates non-specific signal. The membrane was blotted against β-actin to control for protein loading. (C) The 31-kDa band (indicated by a red arrow) was detected in spleen using a monoclonal antibody directed against the N-terminal part of the FAH (green arrow).

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BMC Molecular Biology

ISSN: 1471-2199

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