Figure 8

Steady-state levels of HSP83 transcripts fluctuate during promastigote-to-amastigote differentiation. L. infantum promastigotes were cultured under amastigote growth conditions (panel A) or incubated at 37°C (panel B). RNA samples were prepared at various time points within the treatments (0, 2, 9 and 24 h), transferred onto a nylon membrane and hybridized with the HSP83 3'-UTR probe. C, pXCATIRs-transfected promastigotes were subjected to amastigote growth conditions for 0, 2, 9 and 24 h. The Northern blot was hybridized with the cat probe, and subsequently with the T. cruzi α-tubulin. Bottom panels show methylene blue staining of blots. The autoradiographs were quantified by densitometric scanning and normalized to the intensity at 26°C (arbitrary value 1). The data (relative increase) correspond to mean values derived from at least two independent experiments.