Figure 4
The translation of HSP83 is enhanced in L. infantum promastigotes by incubation at 37°C and 39°C. L. infantum promastigotes were grown at either 26°C, 37°C, 39°C, or 41°C for 2 h in the presence of [35S]methionine/cysteine. Protein samples corresponding to 106 cells were separated over 10% SDS-PAGE and the gel was stained with Coomassie blue (panel A) and, subsequently, the labeled protein bands were revealed by autoradiography (panel B). On panel B, the position of putative HSP83 and HSP70 bands is indicated by arrows. In parallel, lysates from [35S]-labeled promastigotes incubated at the different temperatures were used to immunoprecipitate the HSP83 with specific antibodies. Immunoprecipitates were subjected to SDS-PAGE (panel C). After drying, the autoradiography of the gel was obtained (panel D). The ratio (expressed as relative increase) between incorporation of [35S] and the intensity of the protein band (stained by Coomassie blue) was determined. The data are averages of three independent experiments.