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Figure 1 | BMC Molecular Biology

Figure 1

From: The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation

Figure 1

Temperature-dependent accumulation of transcripts for HSP83 and HSP70. RNA samples were prepared from promastigotes grown at 26°C (lanes 1) or incubated for 2 h at 37°C (lanes 2), 39°C (lanes 3), or 41°C (lanes 4), and analyzed by Northern blotting. Northern blots were hybridized with the L. infantum HSP83 3'-UTR (panel A). The same blot was stripped and rehybridized with probes for the L. infantum HSP70 3'-UTR-I (panel B) and the L. infantum HSP70 3'-UTR-II (panel C) [9]. Panel D shows an ethidium bromide staining of the RNA samples loaded on agarose gel prior to transfer.

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BMC Molecular Biology

ISSN: 1471-2199

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