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Table 4 Bacterial strains used in this study

From: Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

Strain

Relevant Genotype

Source, reference, or construction

AB1157 Backgrounda

KM22

Δ(recC-ptr-recB-recD)::P lac -gam-bet-exo-kan

Murphy, 1998

KM32

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cat

Poteete et al., 1999

TP507

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822

"

TP523

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 ruvC53 eda::Tn 10

Poteete and Fenton, 2000

TP527

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ(srl-recA)306::Tn10

"

TP532

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 recG258::kan Δ(srl-recA)306::Tn 10

"

TP540

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  ruvAB6203::tet

"

TP554

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202

"

TP555

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 ruvC53 eda::Tn10

"

TP559

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ ruvAB6203::tet

"

TP606

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet

"

TP607

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet

"

TP608

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet lexA71::Tn5

"

TP609

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet lexA71::Tn5

"

TP614

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet recO1504::Tn5

"

TP615

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet recF400::Tn5

"

TP625

Δ (recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet recR252::Tn10–9kan

"

TP626

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet recO1504::Tn5

"

TP627

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet recR252::Tn10–9kan

"

TP628

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet recF400::Tn5

"

TP638

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recQ6216::tet

"

TP639

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ recQ6216::tet

"

TP839

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  hsdR::tet

TP507 × PCR productb

TP842

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 Δ  hsdR::tet

TP849 × P1(TP839)

TP849

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978

KM22 × pTP978 linearb

TP850

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 Δ  recA6207::tet

TP849 × P1(TP796)

TP856

Δ(recC-ptr-recB-recD)::P tac -gam-cI996

KM32 × pTP996 linearb

MG1655 Background

TP798

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cat

MG1655 × P1(KM32)

TP829

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822

TP798 × pTP822 linearb

TP832

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979

TP798 × pTP979 linearb

TP872

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979  Δ  lac::cat

TP832 × PCR productb

TP890

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  lac::cat

TP829 × P1(TP872)

TP894

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 lac-inv

TP890 × pTP1034 linearb

TP896

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979 lac-inv

TP872 × pTP1034 linearb

MDS12 Backgroundc

TP750

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822

Poteete, 2004

TP796

Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recA6207::tet

"

  1. a. These strains are presumed to bear all the other genetic markers of AB1157 (Bachmann BJ: Derivations and genotypes of some mutant derivatives of Escherichia coli K-12. In: Escherichia coli and Salmonella: cellular and molecular biology. Edited by Neidhardt FC, III RC, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE. Washington, D.C.: ASM Press; 1996: 2460–2488).
  2. b. Plasmids and PCR products used in the construction of some of the strains are described in the Methods section.
  3. c. MDS12 is a derivative of wild type E. coli strain MG1655 bearing 12 large deletions (Kolisnychenko et al., 2002).