Skip to main content

Table 4 Bacterial strains used in this study

From: Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

Strain Relevant Genotype Source, reference, or construction
AB1157 Backgrounda
KM22 Δ(recC-ptr-recB-recD)::P lac -gam-bet-exo-kan Murphy, 1998
KM32 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cat Poteete et al., 1999
TP507 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 "
TP523 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 ruvC53 eda::Tn 10 Poteete and Fenton, 2000
TP527 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ(srl-recA)306::Tn10 "
TP532 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 recG258::kan Δ(srl-recA)306::Tn 10 "
TP540 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  ruvAB6203::tet "
TP554 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 "
TP555 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 ruvC53 eda::Tn10 "
TP559 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ ruvAB6203::tet "
TP606 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet "
TP607 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet "
TP608 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet lexA71::Tn5 "
TP609 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet lexA71::Tn5 "
TP614 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet recO1504::Tn5 "
TP615 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet recF400::Tn5 "
TP625 Δ (recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  sulA6209::tet recR252::Tn10–9kan "
TP626 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet recO1504::Tn5 "
TP627 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet recR252::Tn10–9kan "
TP628 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ sulA6209::tet recF400::Tn5 "
TP638 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recQ6216::tet "
TP639 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recG6202 Δ recQ6216::tet "
TP839 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  hsdR::tet TP507 × PCR productb
TP842 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 Δ  hsdR::tet TP849 × P1(TP839)
TP849 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 KM22 × pTP978 linearb
TP850 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cI978 Δ  recA6207::tet TP849 × P1(TP796)
TP856 Δ(recC-ptr-recB-recD)::P tac -gam-cI996 KM32 × pTP996 linearb
MG1655 Background
TP798 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-cat MG1655 × P1(KM32)
TP829 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 TP798 × pTP822 linearb
TP832 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979 TP798 × pTP979 linearb
TP872 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979  Δ  lac::cat TP832 × PCR productb
TP890 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  lac::cat TP829 × P1(TP872)
TP894 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 lac-inv TP890 × pTP1034 linearb
TP896 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-bla979 lac-inv TP872 × pTP1034 linearb
MDS12 Backgroundc
TP750 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Poteete, 2004
TP796 Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 Δ  recA6207::tet "
  1. a. These strains are presumed to bear all the other genetic markers of AB1157 (Bachmann BJ: Derivations and genotypes of some mutant derivatives of Escherichia coli K-12. In: Escherichia coli and Salmonella: cellular and molecular biology. Edited by Neidhardt FC, III RC, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE. Washington, D.C.: ASM Press; 1996: 2460–2488).
  2. b. Plasmids and PCR products used in the construction of some of the strains are described in the Methods section.
  3. c. MDS12 is a derivative of wild type E. coli strain MG1655 bearing 12 large deletions (Kolisnychenko et al., 2002).