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Table 2 Recombinant formation by λ lac::cat variants

From: Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

phage

 

description

host

genotypea

% recombinant

% Lac+

181

λ lac::cat819

cut both sides

507

wild

0.54 ± 0.06

7

197

λ lac::cat930

cut right

507

wild

0.92 ± 0.04

10

198

λ lac::cat931

cut left

507

wild

2.8 ± 0.07

0.2

196

λ lac::cat929

cut neither side

507

wild

0.055 ± 0.001

48

196

λ lac::cat929

cut neither side

839

hsdR

0.039 ± 0.002

29

181

λ lac::cat819

cut both sides

554

recG

0.93 ± 0.08

7

197

λ lac::cat930

cut right

554

recG

3.7 ± 0.04

13

198

λ lac::cat931

cut left

554

recG

4.6 ± 0.2

0.2

196

λ lac::cat929

cut neither side

554

recG

0.10 ± 0.03

47

186

λ lac::cat819 nintet

 

507

wild

0.28 ± 0.04

4

186

λ lac::cat819 nintet

 

849

pae

0.024 ± 0.003

45

186

λ lac::cat819 nintet

 

842

pae hsdR

0.027 ± 0.005

49

186

λ lac::cat819 nintet

 

850

pae recA

0.001 ± 0.002

95

186

λ lac::cat819 nintet

 

856

pae red

0.008 ± 0.008

52

208

λ cat988 nintet

right flank only

849

pae

0.008 ± 0.003

58

209

λ cat989 nintet

left flank only

849

pae

0.003 ± 0.0002

100

211

λ cat995 nintet

no flank

849

pae

0.004 ± 0.0002

100

195

λ lac::cat921

short flank

507

wild

0.002 ± 0.001

30

195

λ lac::cat921

short flank

554

recG

0.002 ± 0.0002

50

  1. Bacterial strains were grown to stationary phase without active aeration, and infected at a multiplicity of 0.1 with the indicated phages. Recombination frequencies represent the percentages of infected cells which became chloramphenicol-resistant recombinants; % Lac+ refers to the percentage of chloramphenicol-resistant recombinants which were Lac+. Means and standard errors for at least three measurements are shown. a. All strains have the Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 substitution, except as noted.