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Table 2 Recombinant formation by λ lac::cat variants

From: Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

phage   description host genotypea % recombinant % Lac+
181 λ lac::cat819 cut both sides 507 wild 0.54 ± 0.06 7
197 λ lac::cat930 cut right 507 wild 0.92 ± 0.04 10
198 λ lac::cat931 cut left 507 wild 2.8 ± 0.07 0.2
196 λ lac::cat929 cut neither side 507 wild 0.055 ± 0.001 48
196 λ lac::cat929 cut neither side 839 hsdR 0.039 ± 0.002 29
181 λ lac::cat819 cut both sides 554 recG 0.93 ± 0.08 7
197 λ lac::cat930 cut right 554 recG 3.7 ± 0.04 13
198 λ lac::cat931 cut left 554 recG 4.6 ± 0.2 0.2
196 λ lac::cat929 cut neither side 554 recG 0.10 ± 0.03 47
186 λ lac::cat819 nintet   507 wild 0.28 ± 0.04 4
186 λ lac::cat819 nintet   849 pae 0.024 ± 0.003 45
186 λ lac::cat819 nintet   842 pae hsdR 0.027 ± 0.005 49
186 λ lac::cat819 nintet   850 pae recA 0.001 ± 0.002 95
186 λ lac::cat819 nintet   856 pae red 0.008 ± 0.008 52
208 λ cat988 nintet right flank only 849 pae 0.008 ± 0.003 58
209 λ cat989 nintet left flank only 849 pae 0.003 ± 0.0002 100
211 λ cat995 nintet no flank 849 pae 0.004 ± 0.0002 100
195 λ lac::cat921 short flank 507 wild 0.002 ± 0.001 30
195 λ lac::cat921 short flank 554 recG 0.002 ± 0.0002 50
  1. Bacterial strains were grown to stationary phase without active aeration, and infected at a multiplicity of 0.1 with the indicated phages. Recombination frequencies represent the percentages of infected cells which became chloramphenicol-resistant recombinants; % Lac+ refers to the percentage of chloramphenicol-resistant recombinants which were Lac+. Means and standard errors for at least three measurements are shown. a. All strains have the Δ(recC-ptr-recB-recD)::P tac -gam-bet-exo-pae-cI822 substitution, except as noted.