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Table 1 Genetic requirements of Lac+ recombinant formation

From: Chromosomal duplications and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12

Strain Relevant genotype Recombination % Lac+
recG+ background   
507 wild type 1.00 ± 0.16 41
527 recA 0.01 ± 0.01 42
638 recQ 0.05 ± 0.02 37
540 ruvAB 0.34 ± 0.06 30
523 ruvC 0.22 ± 0.08 29
606 sulA 1.00 ± 0.31 40
608 sulA lexA 0.51 ± 0.01 44
615 sulA recF 0.12 ± 0.09 51
614 sulA recO 0.06 ± 0.00 42
625 sulA recR 0.03 ± 0.02 61
recGΔ background   
554 wild type 1.00 ± 0.13 28
532 recA 0.01 ± 0.01 37
639 recQ 0.05 ± 0.03 46
559 ruvAB 0.34 ± 0.25 41
555 ruvC 0.08 ± 0.02 10
607 sulA 1.00 ± 0.14 37
609 sulA lexA 0.29 ± 0.12 22
628 sulA recF 0.03 ± 0.03 58
626 sulA recO 0.01 ± 0.01 21
627 sulA recR 0.02 ± 0.01 34
  1. Bacterial strains were grown to log phase and infected at a multiplicity of 10 with λ lac::cat819 nin5. Infected cells were aerated for 1 hour at 37°C, then plated on LB agar and LB agar supplemented with chloramphenicol, isopropylthiogalactopyranoside, and X-Gal. Recombination frequencies represent the ratio of chloramphenicol-resistant recombinants to total cell titer, normalized to the ratio of the strain at the top of each group of strains. Means and standard errors for at least three measurements are shown. Actual frequencies were 507, 2.49%; 606, 2.50%, 554, 7.76%; 607, 7.81%. The frequencies of Lac- recombinants in these crosses were published previously (Poteete and Fenton, 2000).