Figure 5

Normal and inverted linear DNA-by-chromosome crosses. Plasmids bearing the indicated lac::cat alleles (corresponding to the phages in Figure 4; the numbers of the plasmids bearing these alleles are given in the Methods section) were digested with BamHI and XhoI (PaeR7 isoschizomer), and electroporated into wild type and lac-inv bacteria (strains TP832 and TP896). Sequence segments are labeled as in Figure 3. The percentages of Lac+ bacteria among the chloramphenicol-resistant recombinant progeny are indicated, as the means and standard errors from two measurements. The total yields of chloramphenicol-resistant recombinants ranged from 20,000 to 200,000 per pmol.