Table 1 Two-hybrid analysis. Prey and bait proteins were expressed as Gal4 AD and LexA BD fusions from pACT2 and pBTM116 respectively. Positive interactions corresponded to 50–100 Miller units (++) or 10–20 Miller units (+) of β-galactosidase activity. Negative interactions corresponded to < 2 units. See text for details.
Prey | Bait | Bridge | Interaction |
|---|---|---|---|
A. Fission yeast proteins | |||
Cdc27 | - | - | - |
- | Pol1(278–527) | - | - |
Cdc27 | Pol1(278–527) | - | ++ |
Pcn1 | Pol1(278–527) | - | - |
Pcn1 | - | Cdc27 | - |
- | Pol1(278–527) | Cdc27 | - |
Pcn1 | Pol1(278–527) | Cdc27 | + |
B. Human proteins | |||
- | Pol α (291–540) | - | - |
p66 (253–466) | - | - | - |
p66 (356–466) | - | - | - |
p66 (253–466) | Pol α (291–540) | - | ++ |
p66 (356–466) | Pol α (291–540) | - | ++ |
C. Cross-species interactions | |||
- | Pol1(278–527) | - | - |
p66 (253–466) | Pol1(278–527) | - | ++ |
p66 (356–466) | Pol1(278–527) | - | ++ |
Cdc27 | Pol α (291–540) | - | - |
D. Truncated Pol1 proteins | |||
Cdc27 | Pol1(278–527) | - | ++ |
Cdc27 | Pol1(328–527) | - | - |
Cdc27 | Pol1(278–477) | - | - |
Cdc27 | Pol1(278–487) | - | - |
Cdc27 | Pol1(278–497) | - | - |
Cdc27 | Pol1(278–507) | - | - |
Cdc27 | Pol1(328–477) | - | - |
Cdc27 | Pol1-TS13(278–527) | - | - |