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Table 1 Two-hybrid analysis. Prey and bait proteins were expressed as Gal4 AD and LexA BD fusions from pACT2 and pBTM116 respectively. Positive interactions corresponded to 50–100 Miller units (++) or 10–20 Miller units (+) of β-galactosidase activity. Negative interactions corresponded to < 2 units. See text for details.

From: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27

Prey Bait Bridge Interaction
A. Fission yeast proteins    
Cdc27 - - -
- Pol1(278–527) - -
Cdc27 Pol1(278–527) - ++
Pcn1 Pol1(278–527) - -
Pcn1 - Cdc27 -
- Pol1(278–527) Cdc27 -
Pcn1 Pol1(278–527) Cdc27 +
B. Human proteins    
- Pol α (291–540) - -
p66 (253–466) - - -
p66 (356–466) - - -
p66 (253–466) Pol α (291–540) - ++
p66 (356–466) Pol α (291–540) - ++
C. Cross-species interactions    
- Pol1(278–527) - -
p66 (253–466) Pol1(278–527) - ++
p66 (356–466) Pol1(278–527) - ++
Cdc27 Pol α (291–540) - -
D. Truncated Pol1 proteins    
Cdc27 Pol1(278–527) - ++
Cdc27 Pol1(328–527) - -
Cdc27 Pol1(278–477) - -
Cdc27 Pol1(278–487) - -
Cdc27 Pol1(278–497) - -
Cdc27 Pol1(278–507) - -
Cdc27 Pol1(328–477) - -
Cdc27 Pol1-TS13(278–527) - -