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Figure 4 | BMC Molecular Biology

Figure 4

From: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27

Figure 4

Mutational analysis of Pol1 binding domain on Cdc27. A. Summary schematic of minimal Pol1 binding region on Cdc27, showing conserved amino acids (boxed) and the eight mutant alleles P1 through P7 and Q1. Each mutant sees two or three adjacent residues being replaced with the same number of alanines. In the case of P1 and P3 through P7, adjacent basic (P3, P4 and P5) or acidic (P1, P6 and P7) amino acids are mutated. B. Quantitation of β-galactosidase activity in liquid cultures. Key: C (pACT2 vector); W (pACT-Cdc27-273-352), mutants P1 – P7, plus Q1 (mutated forms of pACT-Cdc27-273-352); F (pACT-Cdc27, i.e. full-length Cdc27 fused to Gal4).

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BMC Molecular Biology

ISSN: 1471-2199

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