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Figure 1 | BMC Molecular Biology

Figure 1

From: Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27

Figure 1

Mapping of the Pol1 interaction site on Cdc27 using the two-hybrid system. Full-length and thirteen truncated Cdc27 proteins, expressed as Gal4 transcription activation domain fusions, were tested for their ability to interact with Cdc1-LexA, Pcn1-LexA and Pol1(278–512)-LexA baits in S. cerevisiae CTY10-5d. Interactions were monitored by β-galactosidase activity, as described in Material and methods, and are indicated as +++ (strong interaction, typically 80–100 Miller units of β-galactosidase activity), + (weak interaction, <20 Miller units), – (no interaction detectable above background, <1 Miller unit). The area between the broken vertical lines indicates the minimal Pol1 binding region, corresponding to amino acids 293–332 of Cdc27. The grey box indicates the minimal Cdc1 binding domain, amino acids 1–160; the black box represents the Pcn1 (PCNA) binding motif (amino acids 362–372).

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