Figure 4
From: The Drosophila methyl-DNA binding protein MBD2/3 interacts with the NuRD complex via p55 and MI-2
Analysis of the architecure of NuRD (A) Yeast colonies growing on non-selective plates carrying bait (left panel) and prey (top panel) NuRD and MBD2/3 constructs. (B) Replica plate with high-selective medium. Only yeast colonies with constructs encoding interacting proteins are able to grow. MBD2/3liΔ59-339 represents a truncated MBD2/3li construct that was used to confirm the specificity of the interaction between p55 and MBD2/3. (C) X-Gal staining of yeast colonies to confirm interactions. (D) Schematic illustration of protein-protein interactions in the NuRD complex. Homodimerization of p55 and MBD2/3 are not shown.