Real-time PCR quantitation of glucocorticoid receptor alpha isoform

BMC Molecular Biology

Table 1 Evaluation of the standard-curve stability on five different sets of standard constructs.

Pearson's coefficients of correlation of GRα and BCR standard-curves for the same pair of experiments are expressed in the upper and lower rows, respectively. A to E refer to different sets of standard constructs, from Jurkat cell culture, viable cell count, RNA extraction, cDNA synthesis, standard dilution and qrt-PCR for GRα and BCR with calculations.

ISSN: 1471-2199