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Table 1 Evaluation of the standard-curve stability on five different sets of standard constructs.

From: Real-time PCR quantitation of glucocorticoid receptor alpha isoform

  A B C D E
A 1.000 0.993 0.996 0.994 0.993
  1.000 1.000 0.994 0.997 0.996
B 0.993 1.000 0.987 0.981 1.000
  1.000 1.000 0.994 0.997 0.996
C 0.996 0.987 1.000 0.999 0.989
  0.994 0.994 1.000 0.998 0.997
D 0.994 0.981 0.999 1.000 0.982
  0.997 0.997 0.998 1.000 1.000
E 0.993 1.000 0.989 0.982 1.000
  0.996 0.996 0.997 1.000 1.000
  1. Pearson's coefficients of correlation of GRα and BCR standard-curves for the same pair of experiments are expressed in the upper and lower rows, respectively. A to E refer to different sets of standard constructs, from Jurkat cell culture, viable cell count, RNA extraction, cDNA synthesis, standard dilution and qrt-PCR for GRα and BCR with calculations.