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Figure 3 | BMC Molecular Biology

Figure 3

From: Transcriptional oscillation of canonical clock genes in mouse peripheral tissues

Figure 3

Transcriptional oscillation of Bmal1 and Per2 Transcriptional oscillation of Bmal1 (red) and Per2 (blue) was monitored by using a cell culture-based luminescence reporter assay. NIH3T3 cells were transfected with the hBmal1 -Luc or mPer2 -Luc constructs and then stimulated with a high concentration of serum. After the serum shock, in the presence of luciferin, light emission was measured and integrated for 1 min at intervals of 15 min. Ordinate and abscissa represent relative counts and time after serum shock, respectively. The peak of the count was set to 1. 1440 minutes = 1 day.

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