Figure 7
From: Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator
Bel1/Tas interacts with GST-C/H1 fusion proteins detected by GST pull-down assays. The recombinant GST-C/H1 and GST-ΔC/H1-KIX proteins were expressed in E. coli BL21 cells and purified by binding to glutathione Sepharose 4B. Each GST-fusion protein bound to glutathione Sepharose 4B was separately mixed with lysates obtained from pbel1s-transfected 293T cells. After incubation and extensive washing with the binding buffer, bound proteins were eluted, separated by SDS-PAGE, and visualized by staining (upper panel); immunoblotting was carried out with an antibody against Bel1/Tas protein (lower panel).