Figure 6
From: Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator
Mapping of the p300 Bel1/Tas-interaction domain. The p300 expression plasmids, constructs no. 2, and 5 to 8 shown in Fig. 1 were analyzed by immunoprecipitation as described above for p300N. The right hand panel shows the results of immunoblotting obtained after reaction with the monoclonal antibody against the FLAG epitope; arrows and asterisks mark the Bel1/Tas-interacting protein bands from cellular extracts of pbel1s-cotransfected cells (lanes 1–3); intentionally overexposed to visualize the marked bands in lanes 1 and 2. The left panel presents the Western blots of the five recombinant p300N plasmids used for separate co-immunoprecipitations. Numbers in brackets refer to Fig 1.