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Figure 5 | BMC Molecular Biology

Figure 5

From: Coactivators p300 and PCAF physically and functionally interact with the foamy viral trans-activator

Figure 5

Analysis of physical interaction of Bel1/Tas with truncated forms of p300. After cotransfection of 293T cells with 2 μg pbel1s, and, separately with 2 μg each of p300N, p300M, and p300C expression plasmids, cellular extracts were prepared in parallel, divided into equal parts, and analyzed by co-immunoprecipitation and Western blotting. Lysates were precipitated with anti Bel1/Tas antibody and the Western blot was developed with a monoclonal anti-FLAG antiserum. The three arrows in the middle panel mark the correct sizes of the p300N, p300M, and p300C proteins (two blots pasted together). Controls for each p300 plasmid without pbel1s are in lanes 2, 4, and 6. Lower part confirms that the p300N protein specifically binds the Bel1/Tas protein (lane1, marked by arrow); two blots pasted together.

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