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Figure 9 | BMC Molecular Biology

Figure 9

From: Glycogen Synthase Kinase-3 regulates IGFBP-1 gene transcription through the Thymine-rich Insulin Response Element

Figure 9

CHIR99021 does not affect FOXO-1 transactivation potential. H4IIE cells were transfected with BP-1WT along with pEBG2T or pEBG2T-FOXO-1. Cells were incubated with 10 nM insulin or 2 μM CHIR99021 for 24 h prior to lysis and luciferase assays as described in materials and methods. Results are presented as % luciferase activity relative to basal (serum free) luciferase expression and are the means ± S.E. of two experiments performed in triplicate. NS; not significant.

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