Figure 7

CHIR99021 regulates both β-catenin activity and TIRE containing promoter activity. H4IIE cells were transfected with TOPFlash (A) or alternatively with BP-1 WT or BP-1 DM5 (B) reporter constructs. Cells were incubated for 24 h with 10 nM insulin or CHIR99021 at the concentrations shown, prior to lysis and luciferase assays as described in materials and methods. Results are presented as fold induction relative to basal luciferase activity (no inhibitor) (A) or % luciferase activity relative to basal (serum free) luciferase expression (B) and are the means ± standard error of at least two experiments performed in triplicate. The basal activity of BP-1 WT and BP-1 DM5 is not significantly different.