Figure 5

Alsterpaullone does not affect the regulatory phosphorylation sites of PKB, FOXO-1, MAPK, and components of the mTOR pathway. H4IIE cells were serum starved overnight prior to incubation with 10 nM insulin, or alsterpaullone at the concentrations shown for 30 min (A) or 3 h (B). Cells were lysed, and the lysates subjected to SDS PAGE, transferred to nitrocellulose and immunoblotted with antibodies as labelled (Phospho; phosphospecific antibody). Similar results were obtained from two experiments carried out in duplicate