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Figure 3 | BMC Molecular Biology

Figure 3

From: Glycogen Synthase Kinase-3 regulates IGFBP-1 gene transcription through the Thymine-rich Insulin Response Element

Figure 3

Inhibition of GSK-3 does not affect the phosphorylation of MAPK or regulation of the mTOR pathway. H4IIE cells were serum starved overnight prior to incubation with insulin, 10 nM; lithium chloride, 20 mM; SB216763, 30 μM; or SB415286, 100 μM for 15 min (A) or 3 h (B). Cells were lysed, and the lysates subjected to SDS PAGE as described in materials and methods, transferred to nitrocellulose and immunoblotted with antibodies as labelled (Phospho; phosphospecific antibody). Similar results were obtained from two experiments carried out in duplicate

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