Figure 2

SB216763 and SB415286 reduce IGFBP-1 gene expression. H4IIE cells were starved overnight prior to a 3 h incubation with insulin, 10 nM ; dexamethasone, 500 nM; plus or minus SB216763 (A) or SB415286 (B) at the concentrations shown. Total cellular RNA was isolated and an RNase protection assay was performed, as described in material and methods. Results are presented as fold induction relative to control (serum free) and are means ± standard error of two experiments performed in duplicate (upper panels). Representative experiments are also shown (lower panels). ***, p < 0.001 and * p < 0.05