Figure 6

Co-immunoprecipitation of a) p68 and b) p72 with HDAC1. a) HDAC1 was immunoprecipitated from U2OS nuclear extracts using an HDAC1-specific antibody. Immunoprecipitated proteins were separated by SDS-PAGE and the presence of HDAC1 and associated p68 was detected by western blotting with p68- and HDAC1-specific antibodies. b) HDAC1 was immunoprecipitated from nuclear extracts of 293 cells expressing myc-tagged p72 using an HDAC1-specific antibody. Immunoprecipitated proteins were separated by SDS-PAGE and the presence of HDAC1 and associated myc-tagged p72 was detected by western blotting with HDAC1- and myc epitope- specific antibodies. In both experiments a control immunoprecipitation (IP) was performed using an irrelevant rabbit IgG. An aliquot of nuclear extract (NE) was also included in the western blots (west.) as an additional control. H denotes cross reaction with the antibody heavy chain. Molecular weight markers (in kDa) are indicated.