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Figure 2 | BMC Molecular Biology

Figure 2

From: The p53-inhibitor Pifithrin-α inhibits Firefly Luciferase activity in vivo and in vitro

Figure 2

PFT-α does not inhibit Renilla luciferase or CAT reporters in vivo. (A) PFT-α does not inhibit Renilla luciferase. The indicated cell lines were transfected with 1.5 μg of pRL-CMV-luciferase reporter and treated with 20μM PFT-α for 24 hours. Cells were harvested and luciferase activity was measured and normalised for total protein. Results are expressed as fold repression above untreated transfected cells, and represent mean plus standard deviation of a minimum of 3 independent experiments. (B) PFT-α does not inhibit CAT reporter plasmids. U-2 OS cells were transfected with 5 μg of indicated reporters and treated with 20 μM PFT-α for 24 hours. Cells were harvested and CAT activity was measured and normalised for total protein. Results are expressed as percentage of conversion. Since there was a significant variation in the levels of activity of these reporters even in unstimulated conditions, although the overall conclusions were the same, the data is presented is a single experiment representative of 3 independent experiments.

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