Interactions within the mammalian DNA methyltransferase family

BMC Molecular Biology

Table 1 Overview of all tested interactions within the Dnmt family.

	cloned in pACT2	cloned in pBMT117c.1	lacZ	HIS3	Comments
	Dnmt 2	N-Dnmt 1	-	-
	N-Dnmt 3a	C-Dnmt 1	-	-
	C-Dnmt 3a	N-Dnmt 1	-	-
Dnmt 1	N-Dnmt 3b	C-Dnmt 1	-	-
	C-Dnmt 3b	N-Dnmt 1	-	-
vs	DNMT 3L	C-Dnmt 1	-	-
	DNMT 3L	N-Dnmt 1	(+/+)	-	weak autoactivation
	DNMT 3L	control	(+/+)	-	weak autoactivation
other Dnmt's	N-Dnmt 1	DNMT 3L	-	-
	N-Dnmt 1s	DNMT 3L	-	-	short isoform Dnmt1
	DNMT 3L	N-Dnmt 1s	(+)	-	weak autoactivation
	Dnmt 2	C-Dnmt 1	-	-
Dnmt 3a	control	C-Dnmt 3a	-	-
	control	C-Dnmt 3b	-	-
vs	C-Dnmt 3a	N-Dnmt 3a	-	-
	C-Dnmt 3b	N-Dnmt 3b	-	-
Dnmt 3b	N-Dnmt 3a	C-Dnmt 3a	-	-
	N-Dnmt 3b	C-Dnmt 3b	-	-
	DNMT 3L	control	(+/+)	-	weak autoactivation
	control	C-Dnmt 3a	-	-
DNMT 3L	control	C-Dnmt 3b	-	-
	DNMT 3L	N-Dnmt 3a	(+)	-	weak autoactivation
vs	DNMT 3L	N-Dnmt 3b	(+)	-	weak autoactivation
	DNMT 3L	C-Dnmt 3a +++/+++		++/++
other Dnmt's	C-Dnmt 3a	DNMT 3L	+++	+++
	DNMT 3L	C-Dnmt 3b	+/+	-
	C-Dnmt 3b	DNMT 3L	+++	+++
	Dnmt 2	DNMT 3L	-	-
Dnmt 2	Dnmt 2	control	-	-
	Dnmt 2	N-Dnmt 1	-	-
vs	Dnmt 2	C-Dnmt 1	-	-
	Dnmt 2	N-Dnmt 3a	-	-
other Dnmt's	Dnmt 2	N-Dnmt 3b	-	-
	Dnmt 2	DNMT 3L	-	-
	MBD 2	MBD 3	++	++	positive control
	MBD 2	N-Dnmt 1	-	-
MBD2	MBD 2	C-Dnmt 1	-	-
	MBD 2	N-Dnmt 3a	-	-
vs	MBD 2	N-Dnmt 3b	-	-
	MBD 2	DNMT 3L	-	-
Dnmt's	Dnmt 2	MBD 2	++	+++	strong autoactivation
	Dnmt 2	MBD 2	++	+++	strong autoactivation
	control	MBD 2	++	+++	strong autoactivation
MBD3	MBD 3	N-Dnmt 1	-	-
	MBD 3	C-Dnmt 1	-	-
vs	MBD 3	N-Dnmt 3a	-	-
	MBD 3	N-Dnmt 3b	-	-
Dnmt's	MBD 3	DNMT 3L	-	-

The results are grouped in six panels corresponding to the different proteins tested. To allow for completeness within each panel, some of the results are presented in two different panels. N-Dnmt1s corresponds to the N-terminal domain of the short isoform, while the other abbreviations are explained in the text. Duplicate results are separated by a (/).

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