Table 5 Liver damage caused by adenoviruses. The liver lesions were assessed, as described in table 4, two and seven days after the intravenous injection of the adenoviral vector. The total liver inflammation and damage scores range from 0–6 and 0–24 respectively. The transaminase (ALAT) was measured in the serum on day 2 and 7. The normal value of ALAT, measured in ten healthy animals, is 59 +/-19 IU/l. 6 animals per group. The values indicate the mean ± sd
From: Genetic heterogeneity in response to adenovirus gene therapy
High dose (1010 iu) | Low dose (109 iu) | |||
|---|---|---|---|---|
Brown Norway | Wag / Rij | Brown Norway | Wag / Rij | |
Total liver inflammation | ||||
• Day 2 | 0.50 ± 0.31 | 0.41 ± 0.37 | 0.58 ± 0.20 | 0.41 ± 0.20 |
• Day 7 | 3 ± 1.0 | 1.3 ± 0.4 | 1.08 ± 0.49 | 0.66 ± 0.25 |
Total liver damage | ||||
• Day 2 | 2.6 ± 0.5 | 2.1 ± 0.7 | 0.8 ± 0.4 | 0.5 ± 0.2 |
• Day 7 | 14.5 ± 2.6 | 9.3 ± 1.1 | 4.9 ± 0.8 | 2.7 ± 0.7 |
ALAT (IU/l) | ||||
• Day 2 | 63 ± 15 | 58 ± 16 | 44 ± 22 | 55 ± 19 |
• Day 7 | 218 ± 33 | 104 ± 17 | 86 ± 27 | 86 ± 27 |
γGT (IU /l) day 2 | 4.5 ± 0.6 | 4.8 ± 0.5 | Not done | Not done |
LDH (IU /l) day 2 | 837 ± 249 | 741 ± 356 | Not done | Not done |
INF-γ (pg / ml) day 2 | 60 ± 14 | 65 ± 18 | Not done | Not done |
Liver proteins (mg/g tissue) | ||||
• Day 2 | 150 ± 21 | 150 ± 33 | 110 ± 21 | 120 ± 18 |
• Day 7 | 167 ± 32 | 177 ± 31 | 130 ± 42 | 136 ± 25 |
Luciferase (104 RLU/mg protein) | ||||
• Day 2 | 441 ± 158 | 31 ± 27 | 1.2 ± 0.3 | 0.25 ± 0.12 |
• Day 7 | 451 ± 128 | 44 ± 29 | 1.5 ± 0.5 | 0.27 ± 0.17 |