Table 4 Criteria for grading the liver lesions The pathological changes were graded on 3 transversal sections of the right liver lobe. The total damage score is a compilation of scores of apoptosis, vacuolar changes, nuclear condensation, anisokaryosis, megalocytosis, mitosis, and inflammation. All scores are ranging from 0 to 3. The vacuolar change was defined as clear cytoplasm for more the 90% of the nuclear circumference.
From: Genetic heterogeneity in response to adenovirus gene therapy
Score | ||||
|---|---|---|---|---|
0 | 1 | 2 | 3 | |
Apoptosis Number of apoptotic cells / bodies in 10 random 20 × magnification fields | 0–1 | <5 | 5–13 | >13 |
Vacuolar change Percentage of hepatocytes affected. | No or sporadic | 10 % | 10–50% | > 50% |
Condensed nuclei Percentage of hepatocytes affected. | No or sporadic | 10 % | 10–50% | > 50% |
Anisokaryosis | Normal variation. | 5–10% of the nuclei with 2 time the normal size | 10–30% of the nuclei with 2 time the normal size | 10–30% of the nuclei with 3 time the normal size |
Megalocytosis | No or sporadic | 3–10 / 2 sections | > 10 / 2 sections | > 30% of hepatocytes |
Mitosis Number of mitosis in 10 random 20 × magnification fields | 0–1 | <5 | 5–13 | >13 |
Inflammation I Inflammation in the Periportal Space (PS) | Normal background | Few PS with mild inflam. | Many PS with mild inflam. Or Few PS with moderate inflam | Many PS with moderate inflam. Or Few PS with severe inflam. |
Inflammation II Diffuse or multifocal inflammation in sinusoid and central veins | Normal background | Increase of inflammatory cells | Aggregates of inflammatory cells | Aggregates of inflammatory cells obscuring the liver architecture |