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Table 4 Criteria for grading the liver lesions The pathological changes were graded on 3 transversal sections of the right liver lobe. The total damage score is a compilation of scores of apoptosis, vacuolar changes, nuclear condensation, anisokaryosis, megalocytosis, mitosis, and inflammation. All scores are ranging from 0 to 3. The vacuolar change was defined as clear cytoplasm for more the 90% of the nuclear circumference.

From: Genetic heterogeneity in response to adenovirus gene therapy

  Score
  0 1 2 3
Apoptosis Number of apoptotic cells / bodies in 10 random 20 × magnification fields 0–1 <5 5–13 >13
Vacuolar change Percentage of hepatocytes affected. No or sporadic 10 % 10–50% > 50%
Condensed nuclei Percentage of hepatocytes affected. No or sporadic 10 % 10–50% > 50%
Anisokaryosis Normal variation. 5–10% of the nuclei with 2 time the normal size 10–30% of the nuclei with 2 time the normal size 10–30% of the nuclei with 3 time the normal size
Megalocytosis No or sporadic 3–10 / 2 sections > 10 / 2 sections > 30% of hepatocytes
Mitosis Number of mitosis in 10 random 20 × magnification fields 0–1 <5 5–13 >13
Inflammation I Inflammation in the Periportal Space (PS) Normal background Few PS with mild inflam. Many PS with mild inflam. Or Few PS with moderate inflam Many PS with moderate inflam. Or Few PS with severe inflam.
Inflammation II Diffuse or multifocal inflammation in sinusoid and central veins Normal background Increase of inflammatory cells Aggregates of inflammatory cells Aggregates of inflammatory cells obscuring the liver architecture