Figure 1
FMR1 (CGG) n TK-CAT constructs. (a) A schematic representation of the constitutive HSVtk-CAT promoter showing the position of the (CGG)n repeat cloning site. The distance from the start site of transcription to the repeats, in base pairs, is similar as that found in the native FMR1 promoter. The arrow represents the position of the primer used for the analysis of transcripts by primer extension and the thickened line represents pBR322 vector-derived DNA. (b) An agarose gel showing reporter plasmid digests in which Hind III treatment releases the (CGG)n and flanking sequences. Lanes 1, 2 and 3 are digested pBR TK-CAT plasmids containing (CGG)27, (CGG)70 and (CGG)105 repeat lengths respectively. The released fragments are shown by the arrows corresponding to (CGG)105, (CGG)70 and (CGG)27 respectively. The marker lane contains a 1 kb ladder