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Table 4 Nonsense mutations in SUP45 support ascospore viability in presence of SUQ5

From: Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae

Strain D1638 transformed with plasmids:

Total number of tetrads

Number of tetrads with viable spores

% of His+Ura+ segregants

  

4

3

2

1

 

pRS316/SUP45

35

8

12

14

1

32

pRS316/sup45-101

36

11

11

13

1

33

pRS316/sup45-102

31

9

10

11

1

35

pRS316/sup45-104

20

1

7

11

1

17

pRS316/sup45-105

36

6

10

18

2

30

pRS316/sup45-107

36

2

9

24

1

18

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BMC Molecular Biology

ISSN: 1471-2199

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