Table 4 Strains used and constructed in this study
From: Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
Strain | Description | Source or reference |
|---|---|---|
EDL933 | EHEC O157:H7 prototype | [39] |
TUV93-0 | Stx-derivative of EDL933 | A. Donohue-Rolfe |
JPN15/pMAR7 | AmpR derivative of EPEC E2348/69 O127:H6 prototype | [40] |
Derivatives of EHEC TUV93-0: | ||
KM45 | Δtir-cesT-eae::cat | TUV93-0/ pTP223 × Sac I-Sph I fragment from pKM185 |
KM46 | Δtir-cesT-eae::cat-sacB | TUV93-0/ pTP223 × Sac I-Sph I fragment from pKM188 |
KM47 | Δeae::cat | TUV93-0/ pTP223 × Sac I-Sph I fragment from pKM184 |
KM48 | Δeae::cat-sacB | TUV93-0/ pTP223 × Sac I-Sph I fragment from pKM187 |
KM60 | Δeae | KM48/ pTP223 × Sac I-Sph I fragment from pKM181 |
KM61 | Δtir-cesT-eae | KM46/ pTP223 × Sac I-Sph I fragment from pKM182 |
KC2 | Δtir::cat | TUV93-0/pTP223 × Kpn I fragment from pKC2 |
KC3 | Δtir::cat-sacB | TUV93-0/ pTP223 × Kpn I fragment from pKC3 |
KC5 | Δtir | KC3/ pTP223 × Kpn I fragment from pKC5 |
KC27 | Δtir::cat | TUV93-0/pKM201 × PCR fragment (5KO-H-tir, 3KO-H-tir) |
KC30 | ΔLEE::cat | TUV93-0/pKM201 × PCR fragment (5KO-H-LEE, 3KO-H-LEE) |
KC40 | ΔespF::cat | TUV93-0/pKM201 × PCR fragment (5KO-H-espF, 3KO-H-espF) |
KM90 | ΔlacZ::cat | TUV93-O/pKM201 × PCR fragment (cat13, cat14) |
KM94 | ΔlacZ::kan | TUV93-O/pKM208 × PCR fragment (kan3, kan4) |
KM95 | ΔeaeH::kan | TUV93-O/pKM208 × PCR fragment (5KO-H-Island 12, 3KO-H-Island 12) |
KM96 | ΔZ3025-Z3026::kan | TUV93-O/pKM208 × PCR fragment (5KO-H-Island 77, 3KO-H-Island 77) |
KM97 | ΔZ3664::kan | TUV93-O/pKM208 × PCR fragment (5KO-H-Island 103, 3KO-H-Island 103) |
KM98 | ΔhopD-Z4695-yheA::kan | TUV93-O/pKM208 × PCR fragment (5KO-H-Island 130–131, 3KO-H-Island 130–131) |
KM99 | ΔZ58155-Z5816::kan | TUV93-O/pKM208 × PCR fragment (5KO-H-Island 169, 3KO-H-Island 169) |
Derivatives of EPEC JPN15/pMAR7 | ||
KC21 | ΔLEE::cat | JPN15/pMAR7/ pTP223 × PCR fragment (3KO-P-LEE, 5KO-P-LEE) |