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Figure 2 | BMC Molecular Biology

Figure 2

From: Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli

Figure 2

Uncontrolled λ Red expression is mutagenic. A single fresh colony of EHEC strain TUV93-0 (with indicated plasmids) was suspended in 1 ml LB containing 100 μg/ml ampicillin. The cell suspension was diluted with LB-ampicillin to a final concentration of 5 × 104 cells/ml and aliquoted to 24 culture tubes. Overnight cultures (0.3 ml each) of EHEC with control plasmid (circles), pKM201 (squares), pKM208 (triangles) and pKM208 with IPTG added (diamonds) were plated on LB plates containing 100 μg/ml rifampicin. Plates were incubated overnight at 37 degrees and rifampicin resistant colonies were counted 24 hours later. Average number of RifR colonies (with standard errors) for each culture are as follows: control plasmid, 14.1 (2.7); pKM201, 173 (33.3); pKM208, 40.4 (9.9); pKM208 + IPTG, 194 (21.9).

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