Figure 1From: Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coliSchematic of λ Red-promoted PCR-mediated recombination. PCR products, containing a drug marker flanked by 40–60 bp of target DNA, are generated by primers designated 5KO and 3KO (see Table 6 in Additional file #2) and electroporated into EHEC containing pKM201 or pKM208 (or EPEC containing pTP223). Chromosomal replacements were verified by PCR using primers upstream (U), downstream (D) and within the drug marker (M) (data not shown).Back to article page