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Table 1 Evaluation of host context and dependence on phage protein expression in ssOR.

From: Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells

Strain + plasmid Genotype + expressed proteins Normalised ssOR
MM294 wild-type 0
   + pBADET    + RecE, RecT 50
   + pBADETγ    + RecE, RecT, Redγ 46
   + pBADαβ    + Redα, Redβ 55
   + pBADαβγ    + Redα, Redβ, Redγ 52
JC8679 SbcA, recBC 15
JC5519 RecBC 0
   + pBADrecET    +RecE, RecT 49
   + pBADredαβ    + Redα, Redβ 52
   + pBADerf    + Erf 35
JC9366 recA 0
   + pBADrecET    +RecE, RecT 45
   + pBADrecETγ    +RecE, RecT, Redγ 50
   + pBADredαβ    + Redα, Redβ 54
   + pBADredαβγ    + Redα, Redβ, Redγ 51
   +pBADerf    + Erf 32
JM103 sbcBC 0.1
JC9387 sbcBC, rac, recBC 0.3
JC15329 sbcBC, recA, recAB, rac 0
JC8111 sbcBC, rac, recBC, recF 0.1
  1. Various E. coli hosts were transformed with pBAD24 expression plasmids or not as indicated before co-electroporation with a 48 mer oligonucleotide and pGKneoΔ to test for ssOR. The strains used were MM294, JC8679 [44], JC5519 [45], JC9366 [45], JM103 [46], JC 9387 [45], JC15329 [47] and JC8111 [48]. Relevant aspects of genotype and expressed proteins, after arabinose induction, are listed in the central column. Numbers refer to the ratio of kanamycin to ampicillin resistant colonies and were generated from the same master mixture of oligonucleotide and pGKneoΔ.