Table 4 Intracellular concentration (μM) of dinucleoside polyphosphates controlled by the Hnt2 active site
Culture time | 24 hr | 48 hr | Â | ||
|---|---|---|---|---|---|
Nucleotide | ApppN | AppppN | ApppN | AppppN | Â |
Plasmid in strain BY71-6c | Â | Â | Â | Â | Genotype |
None | 20.70 | 0.16 | 129.00 | 2.16 | hnt2Δ |
pRS423 | 23.80 | 0.21 | 140.00 | 1.11 | hnt2Δ |
pB05 | 0.51 | 0.07 | 1.11 | 0.32 | HNT2 |
pB32 | 18.70 | 0.17 | 201.00 | 1.69 | HNT2-His109Ala |
pB86 | 13.40 | 0.16 | 80.40 | 1.00 | HNT2-His109Asp |