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Figure 2 | BMC Molecular Biology

Figure 2

From: A modification of Representational Difference Analysis, with application to the cloning of a candidate in the Reelin signalling pathway

Figure 2

Agarose gel electrophoresis (1.5% NuSieve, FMC) of representations and a first round RDA product DP-1 obtained using the modified protocol. A. Both representations of normal (lane 1) and scrambler (lane 2) brain transcripts (used respectively as driver and tester) show a smear between 200 and 1100 bp. B. After the first round of subtractive hybridization/amplification, product DP-1 obtained using the proposed modified protocol exhibits reduced complexity with visible bands (lane 2). The negative control of the reaction is loaded on lane 1. C. By comparison, the usual RDA protocol yields a smear in the DP1. D. and discrete bands only in the DP2 product (lane2). On lane 1 is the control reaction. The size o the photos was determined using 100 bp DNA marker (Invitrogen).

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