Figure 1
From: Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex
Release of Cdc14 from the nucleolus requires Cdc5. CDC14-HA3 cells in wild type (+) (RJD 1191) or cdc5-1 (RJD 1217) background were grown at 25°C, and a portion of the cultures were further shifted to 33°C for three hours to arrest cdc5-1 in late anaphase. Cells were subjected to indirect immunofluorescence with HA.11 to visualize Cdc14-HA3 (Column 1) and either anti-tubulin or anti-A190 antibodies to visualize the mitotic spindles and the nucleoli, respectively (Column 2). The positions of nuclei, as indicated by DAPI staining, are shown in Column 3.